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Plant Physiology 65:27-32 (1980)
© 1980 American Society of Plant Biologists

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Articles

Some New Aspects of the in Vivo Assay for Nitrate Reductase in Wheat (Triticum aestivum L.) Leaves

I. REEVALUATION OF NITRATE POOL SIZES 1

Richard H. Hageman, Andrew J. Reed, Rise A. Femmer, Joseph H. Sherrard and Michael J. Dalling2

Department of Agronomy, University of Illinois, Urbana, Illinois 61801

Experiments were carried out to clarify problems encountered in measuring metabolic and storage pool sizes of nitrate in wheat leaf sections with an in vivo nitrate reductase assay. The leaf sections were from seedlings grown on 15 millimolar nitrate. Data obtained show that the cessation of nitrite accumulation, used as an index of the active nitrate pool size, could be caused by lack of anaerobiosis in the assay system, the lack of energy for nitrate reduction, or a loss of nitrate reductase activity. Availability of nitrate was never the limiting factor in this system. It is concluded that pool sizes of nitrate cannot be determined in wheat leaves with the in vivo assays employed.

2 Present address: School of Agriculture and Forestry, University of Melbourne, Parkville, Victoria 3052, Australia.

1 This work was supported by a Senior Research Scholarship (RHH) from the Australian-American (Fulbright) Committee and grants from the Wheat Industry Research Committee of Victoria, Australia and Pioneer Hi-Bred International, Inc., Johnston, Iowa.






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Copyright © 1980 by the American Society of Plant Biologists