Plant Physiology 65:57-60 (1980)
© 1980 American Society of Plant Biologists
Articles
Establishment of an Experimental System for the Study of Tracheary Element Differentiation from Single Cells Isolated from the Mesophyll of Zinnia elegans
Hiroo Fukuda and
Atsushi Komamine
Department of Botany, Faculty of Science, University of Tokyo, Tokyo 113 Japan
Single cells were isolated mechanically from the mesophyll of adult plants and of seedlings of Zinnia elegans L. cv. Canary bird. When single cells isolated from the first leaves of seedlings were cultured in a liquid medium in the dark with rotation, they differentiated to tracheary elements with a reasonable degree of synchrony in the 24-hour period between days 2 and 3 after culture. The proportion of tracheary elements as a percentage of total cells reached nearly 30% 3 days after culture. Factors favoring cytodifferentiation were certain optimum levels of both  -naphthalene-acetic acid (0.1 milligram per liter) and benzyladenine (1 milligram per liter), a low concentration of ammonium chloride (0 to 1 millimolar), and an initial cell population density in the range 0.4 to 3.8 x 105 cells/ml. It was possible to follow analytically the sequence of cytodifferentiation in individual cells in this system.
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