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Plant Physiology 65:85-87 (1980)
© 1980 American Society of Plant Biologists

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Articles

Assessment of Cytoplasmic Contaminations in Isolated Vacuole Preparations 1,2

Arie Admon and Benjamin Jacoby

Eliezer E. Goldschmidt

Department of Agricultural Botany, The Hebrew University, Rehovot, Israel, Department of Horticulture, The Hebrew University

A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain "impure vacuoles," namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast, "naked vacuoles," do not stain.

1 This work was supported by grants from the Winick Institute for Soil and Plant Nutrition Research and from the Hebrew University of Jerusalem Research Foundation.

2 Part of a M.Sc. thesis by A. A.






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