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Plant Physiology 65:184-185 (1980)
© 1980 American Society of Plant Biologists

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Articles

Crown Gall Tumor Disc Bioassay

A POSSIBLE AID IN THE DETECTION OF COMPOUNDS WITH ANTITUMOR ACTIVITY

Alan G. Galsky1 and James P. Wilsey

Richard G. Powell

Department of Biology, Bradley University, Peoria, Illinois 61625, Northern Regional Research Center, Agricultural, Research, Science, and Education Administration, Peoria, Illinois 61604

Seventeen samples consisting of purified compounds and various ethanol extracts from plant sources were tested for activity on the initiation of crown gall tumors on potato discs. The results demonstrated definite correlation between the ability of these samples to inhibit the formation of crown gall tumors and their activity on the P388 leukemia system in mice. Samples showing only cytotoxic effects in KB cell cultures did not affect tumor initiation in our system. The active materials had no effects on bacterial viability or on the ability of the bacteria to attach to a tumorbinding site.

1 To whom reprint requests should be sent.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1980 by the American Society of Plant Biologists