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Gibberellin-sensitive Suspension Cultures ¹

Botanical Laboratories, University of Leicester, England

Suspension cultures were incubated in the presence and absence of gibberellic acid (GA₃) in an attempt to define a new experimental system for study of the molecular action of gibberellins upon growth. Unlike many suspension cultures, an auxin-independent green clone from spinach (Spinacia oleracea L.) and an auxin-dependent line of "Paul's Scarlet" rose (Rosa sp.) were promoted in expansion growth by GA₃ at 10^–11 to 10^–6 molar. In Rosa the cells also elongated upon GA₃ treatment whereas in Spinacia they remained isodiametric.

Attempts were made to intensify the response. The effect of GA₃ in Spinacia was stronger when gas exchange between the culture and the laboratory air was facilitated. The response of Rosa was dependent on the presence of a cytokinin, although this culture did not require exogenous cytokinin for serial subculture. GA₃ antagonized the growth retardant AMO-1618 in Rosa but not in Spinacia. In general, conditions that enhanced growth also rendered GA₃ a more effective promoter; conversely, GA₃ tended to become inhibitory under conditions that permitted only slow growth.

³ Deceased.

¹ S.C.F. thanks the Science Research Council for a Studentship during the tenure of which this work was done.

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