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Chlorophyll Determination in Intact Tissues Using N,N-Dimethylformamide

Department of Botany, The George S. Wise Faculty for Life Sciences, Tel Aviv University, Ramat Aviv, Israel

Photosynthetic pigments from etiolated cucumber (Cucumis sativus var. Beit Alpha improved, Hazera Co., Gedera) cotyledons were extracted by direct immersion of the intact cotyledons into the solvent N,N-dimethylformamide (DMF). The solvent is especially efficient when pigment concentration is low; time and tools are saved and the loss of pigment that usually occurs in more complicated extraction procedures is prevented. The specific absorption coefficient of chlorophyll a in DMF was also determined.

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