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Plant Physiology 65:502-505 (1980)
© 1980 American Society of Plant Biologists

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Articles

Nitrate Effect on Nitrogen Fixation (Acetylene Reduction)

ACTIVITIES OF LEGUME ROOT NODULES INDUCED BY RHIZOBIA WITH VARIED NITRATE REDUCTASE ACTIVITIES 1

James R. Manhart and Peter P. Wong

Division of Biology, Kansas State University, Manhattan, Kansas 66506

The effect of nitrate on symbiotic nitrogen fixation by root nodules of cowpea (Vigna unguiculata L., Walp., cv. California Blackeye) and lupine (Lupinus augustifolius L., cv. Frost) plants inoculated with nitrate reductase-expressing and nitrate reductase-nonexpressing Rhizobium strains were examined. Nitrate reductase of Rhizobium bacteroids in the nodules of cowpea and lupine reduced nitrate to nitrite. Both cowpea and lupine nodules accumulated nitrite when grown in the presence of 15 millimolar nitrate and induced by Rhizobium strains which express nitrate reductase activity (Rhizobium sp. 32H1 and 127E15). The nitrogen fixation (acetylene reduction) activities of cowpea and lupine nodules were inhibited by nitrate whether the nodules were induced by Rhizobium strains that express (Rhizobium sp. 32H1 and 127E15) or do not express (Rhizobium sp. 127E14 and R. lupini ATCC 10318) nitrate reductase activity. These findings indicate that nitrite, the product of bacteroid nitrate reductase, may not play a role in the inhibitory effect of nitrate on nitrogen fixation activities of legume root nodules. However, the degree of inhibition on the fixation activity by nitrate varied in different legume-Rhizobium combinations.

1 This research was supported by National Science Foundation Grants PCM76-81214 and PCM76-81214A02, by United States Department of Agriculture, Science and Education Administration, Competitive Grants Office Grant 5901-0410-8-0094-0, and by the Kansas Agricultural Experiment Station (Biology No. 79-360-J).






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Copyright © 1980 by the American Society of Plant Biologists