Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 65:557-559 (1980)
© 1980 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Whatley, M. H.
Right arrow Articles by Sequeira, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Whatley, M. H.
Right arrow Articles by Sequeira, L.
Agricola
Right arrow Articles by Whatley, M. H.
Right arrow Articles by Sequeira, L.
Short Communication

Lipopolysaccharide Composition of the Wilt Pathogen, Pseudomonas solanacearum

CORRELATION WITH THE HYPERSENSITIVE RESPONSE IN TOBACCO 1

Mariamne H. Whatley, Nora Hunter, Michael A. Cantrell, Carol Hendrick, Kenneth Keegstra2 and Luis Sequeira

Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706, 2 Department of Botany, University of Wisconsin, Madison, Wisconsin 53706

In the induction of the hypersensitive response (HR) in tobacco by Pseudomonas solanacearum, the recognition between host and pathogen is thought to involve an interaction between plant lectins and bacterial lipopolysaccharide (LPS). The LPS of a series of strains of P. solanacearum were examined to determine if there are structural differences that might account for the ability or inability of these strains to induce the hypersensitive response. Analysis of the components of LPS by gas chromatography indicates a clear difference in sugar composition between the HR-inducing and non-HR-inducing strains, especially in terms of the percentage of glucose, xylose and rhamnose. Sodium dodecyl sulfate polyacrylamide gel electrophoresis shows there are two distinct kinds of LPS, differing greatly in size, which correspond to rough and smooth LPS in other systems. In addition, a phage, CH154, was isolated which lyses non-HR-inducing bacteria and which is inactivated by LPS from these bacterial strains. Therefore, differences in LPS structure correlate strongly with host recognition of Pseudomonas solanacearum.

1 Supported in part by Rockefeller Foundation Grant 133-C500 and National Science Foundation Grant 144-K487 to L. S. and National Science Foundation National Needs Postdoctoral Fellowship to M. H. W.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
C. M. Rojas, J. H. Ham, W.-L. Deng, J. J. Doyle, and A. Collmer
HecA, a member of a class of adhesins produced by diverse pathogenic bacteria, contributes to the attachment, aggregation, epidermal cell killing, and virulence phenotypes of Erwinia chrysanthemi EC16 on Nicotiana clevelandii seedlings
PNAS, October 1, 2002; 99(20): 13142 - 13147.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1980 by the American Society of Plant Biologists