Plant Physiol.
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Plant Physiology 65:605-609 (1980)
© 1980 American Society of Plant Biologists

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Articles

Oxyleghemoglobin-mediated Hydrogen Oxidation by Rhizobium japonicum USDA 122 DES Bacteroids 1

David W. Emerich, Steve L. Albrecht2, Sterling A. Russell, Temay Ching3 and Harold J. Evans

Laboratory for Nitrogen Fixation Research, Oregon State University, Corvallis, Oregon 97331

Oxyleghemoglobin was used to supply low concentrations of O2 to H2-oxidizing bacteroids from Rhizobium japonicum USDA 122 DES. The H2 oxidation system of these bacteroids was capable of effectively utilizing O2 at the low concentrations of O2 expected to be found in soybean nodules. Apparent Km values of approximately 10 nanomolar O2 have been calculated for the oxyhydrogen reaction. These values include the Km values for both H2 oxidation and endogenous substrate oxidation. Even in the presence of oxyleghemoglobin, H2 additions stimulated C2H2 reduction, reduced the rate of endogenous respiration and maintained the ATP contents of bacteroids. In our reconstituted oxyleghemoglobin and bacteriod system, we estimate that the H2 oxidation system is capable of recycling all of the H2 evolved during the N2 fixation process.

2 Present address: Plant Science Unit, ARS Southern Region, Department of Agronomy, 304 Newell Hall, University of Florida, Gainesville, Florida 32611.

3 Department of Agronomic Crop Science, Oregon State University.

1 This research was supported by the National Science Foundation Grant PCM 77-08784, postdoctoral fellowships to D. W. E. from the Rockefeller Foundation and the National Science Foundation (SP 178-15650), and also the research was supported by the Oregon Agricultural Experiment Station from which this is Technical Paper No. 5263.






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Copyright © 1980 by the American Society of Plant Biologists