Plant Physiol.
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Plant Physiology 65:614-617 (1980)
© 1980 American Society of Plant Biologists

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Measurement of Tissue Osmotic Pressure 1

William Wenkert

United States Department of Agriculture, 2073 Neil Avenue, Columbus, Ohio 43210

Osmotic pressure measured by a modified pressure-volume method was compared with that of the mixed sap expressed from frozen and thawed tissue. The error in the latter technique averaged 11 and 16% (too dilute) for greenhouse and field leaves of Zea mays L. at all growth stages. These errors were not consistently calculated by a model of simple mixing between the matric and osmotic fractions, both of which decreased with plant age. Some other alternatives to the pressure-volume method are discussed which are based on a more rapid estimate of the zero turgor point.

1 Contribution from the Soil Drainage Research Unit, United States Department of Agriculture, Science and Education Administration, Agricultural Research, and the Ohio Agricultural Research and Development Center, Wooster, Ohio. OARDC Journal No. 68-79.






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Copyright © 1980 by the American Society of Plant Biologists