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Identification of Six Endogenous Gibberellins in Spinach Shoots ¹

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Analysis of highly purified extracts from spinach shoots by combined gas chromatography-mass spectrometry has demonstrated the presence of six 13-C-hydroxylated gibberellins (GAs): GA₅₃, GA₄₄, GA₁₉, GA₁₇, GA₂₀, and GA₂₉. The major GAs were GA₁₇, GA₁₉, and GA₂₀, whereas the other three GAs occurred in trace amounts. Structural considerations suggest that the six GAs identified in spinach are related in the following metabolic sequence: GA₅₃ GA₄₄ GA₁₉ GA₁₇ GA₂₀ GA₂₉.

¹ This work was supported by the United States Department of Energy Contract EY-76-C-02-1338.

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