Plant Physiology 65:761-767 (1980)
© 1980 American Society of Plant Biologists
Articles
Biological Activities of Indoleacetylamino Acids and Their Use as Auxins in Tissue Culture 1
Roger P. Hangarter,
Michael D. Peterson2 and
Norman E. Good
Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824
The auxin activities of a number of indoleacetylamino acid conjugates have been determined in three test systems: growth of tomato hypocotyl explants (Lycopersicon esculentum Mill. cv. Marglobe); growth of tobacco callus cultures (Nicotiana tabacum L. cv. Wisconsin 38); and ethylene production from pea stems (Pisum sativum L. cv. Alaska). The activities of the conjugates differ greatly depending on the amino acid moiety. Indoleacetyl-L-alanine supports rapid callus growth from the tomato hypocotyls while inhibiting growth of shoots and roots. Indoleacetylglycine behaves in a similar manner but is somewhat less effective in supporting callus growth and in inhibiting shoot formation. The other amino acid conjugates tested (valine, leucine, aspartic acid, threonine, methionine, phenylalanine, and proline) support shoot formation without supporting root formation or much callus growth. The tobacco callus system, which forms abundant shoots in the presence or absence of free indoleacetic acid, produces only rapid undifferentiated growth in the presence of indoleacetyl-L-alanine and indoleacetylglycine. The other conjugates inhibit shoot formation weakly if at all. Most of the conjugates induce sustained ethylene production from the pea stems but at rates well below the initial rates observed with free indoleacetic acid. Many, but not all of the effects of conjugates such as indoleacetyl-L-alanine can be mimicked by frequent renewals of the supply of free indoleacetic acid.
2 Present address: United States Department of Agriculture, Agricultural Research Service, Potato Introduction Station, Sturgeon Bay, Wisconsin 54235.
1 Michigan Agricultural Experiment Station Journal Article No. 9094. This investigation was supported in part by National Science Foundation Grant PCM-76-07581-A01 to N. E. G. and Department of Energy Grant EY-76-S-02-2528. M005 to Peter S. Carlson.
This article has been cited by other articles:
|
|
|
|
 
P. E. Staswick, B. Serban, M. Rowe, I. Tiryaki, M. T. Maldonado, M. C. Maldonado, and W. Suza
Characterization of an Arabidopsis Enzyme Family That Conjugates Amino Acids to Indole-3-Acetic Acid
PLANT CELL,
February 1, 2005;
17(2):
616 - 627.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Rampey, S. LeClere, M. Kowalczyk, K. Ljung, G. Sandberg, and B. Bartel
A Family of Auxin-Conjugate Hydrolases That Contributes to Free Indole-3-Acetic Acid Levels during Arabidopsis Germination
Plant Physiology,
June 1, 2004;
135(2):
978 - 988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. LeClere, R. A. Rampey, and B. Bartel
IAR4, a Gene Required for Auxin Conjugate Sensitivity in Arabidopsis, Encodes a Pyruvate Dehydrogenase E1{alpha} Homolog
Plant Physiology,
June 1, 2004;
135(2):
989 - 999.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. LeClere, R. Tellez, R. A. Rampey, S. P. T. Matsuda, and B. Bartel
Characterization of a Family of IAA-Amino Acid Conjugate Hydrolases from Arabidopsis
J. Biol. Chem.,
May 31, 2002;
277(23):
20446 - 20452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. T. Davies, D. H. Goetz, J. Lasswell, M. N. Anderson, and B. Bartel
IAR3 Encodes an Auxin Conjugate Hydrolase from Arabidopsis
PLANT CELL,
March 1, 1999;
11(3):
365 - 376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
