This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (50) Citing Articles via Google Scholar Google Scholar Articles by Mozer, T. J. Search for Related Content PubMed PubMed Citation Articles by Mozer, T. J. Agricola Articles by Mozer, T. J.

Articles

Partial Purification and Characterization of the mRNA for -Amylase from Barley Aleurone Layers ¹

Department of Biology, Washington University, St. Louis, Missouri 63130

The poly(A)-containing mRNA from barley aleurone layers pretreated with gibberellic acid has been purified by phenol-chloroform extraction and repeated oligo[d(pT)]-cellulose chromatography. This RNA has been translated in both the wheat germ and reticulocyte lysate in vitro translation systems with greater than 50% of the synthesized protein being -amylase. The mRNA for -amylase has been further purified by dimethylsulfoxide-formamide-sucrose density gradient centrifugation and by gel electrophoresis. By these methods, its molecular weight has been determined to be 580,000.

¹ This work was supported by National Institutes of Health Postdoctoral fellowship 1-F32-GM06663-01, by a BRSG Grant awarded by the Biochemical Research Support Grant Program, Division of Research Resources, National Institutes of Health, by the Department of Biology, Faculty of Arts and Sciences Central Budget, Washington University, and by the Agricultural Products Division of Monsanto Chemical Company.

This article has been cited by other articles:

				J C Larkin, J P Hunsperger, D Culley, I Rubenstein, and C D Silflow The organization and expression of a maize ribosomal protein gene family. Genes & Dev., April 1, 1989; 3(4): 500 - 509. [Abstract] [PDF]