This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Oliver, D. J. Search for Related Content PubMed PubMed Citation Articles by Oliver, D. J. Agricola Articles by Oliver, D. J.

Articles

The Effect of Glyoxylate on Photosynthesis and Photorespiration by Isolated Soybean Mesophyll Cells

Department of Biochemistry, Connecticut Agricultural Experiment Station, P.O. Box 1106, New Haven, Connecticut 06504

Incubating isolated soybean leaf mesophyll cells with glyoxylate increased the rates of CO₂ fixation by as much as 150%. In order to cause this stimulation, the glyoxylate must be presented to the cells before the NaHCO₃. Significant stimulation was observed 15 seconds after beginning the glyoxylate treatment. The glyoxylate-dependent stimulation was increased by high O₂ concentrations and decreased by high CO₂ concentrations. Glyoxylate treatment resulted in a 71% inhibition in the rate of CO₂ incorporation into glycolate and glycine. Glyoxylate may be stimulating net photosynthesis solely by decreasing photorespiration or it may be increasing the amount of CO₂ fixed by both decreasing photorespiration and increasing gross photosynthesis. Ribulose bisphosphate carboxylase, when preactivated and assayed in situ, was unaffected by the glyoxylate treatment.