Plant Physiol.
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Plant Physiology 65:939-943 (1980)
© 1980 American Society of Plant Biologists

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Articles

Synthesis of Nitrate Reductase in Chlorella

I. EVIDENCE FOR AN INACTIVE PROTEIN PRECURSOR 1

Edward A. Funkhouser

Teh-Chien Shen2 and Renate Ackermann

Department of Plant Sciences, Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas 77843, Forschungsstelle Vennesland der Max-Planck-Gesellschaft, Harnackstrasse 23, D-1000 Berlin 33 (Dahlem) Federal Republic of Germany

Synthesis of nitrate reductase (EC 1.6.6.1) in Chlorella vulgaris was studied under inducing conditions, i.e. with cells grown on ammonia and then transferred to nitrate medium. Cycloheximide (but not chloramphenicol) completely inhibited synthesis of the enzyme, but only if it was added at the start (i.e. at the time of nitrate addition) of the induction period. Cycloheximide inhibition became less effective as induction by nitrate proceeded. Enzyme from small quantities of culture (1 to 3 milliliters of packed cells) was purified to homogeneity with the aid of blue dextran-Sepharose chromatography. Incorporation of radioactivity from labeled arginine into nitrate reductase was measured in the presence and absence of cycloheximide. Conditions were found under which the inhibitor completely blocked the incorporation of labeled amino acid, but only slightly decreased the increase in nitrate reductase activity. The results indicate that synthesis of nitrate reductase from amino acids proceeds by way of a protein precursor which is inactive enzymically.

2 Recipient of Alexander von Humboldt-Stiftung fellowship. Permanent address: Department of Botany, University of Malaya, Kuala Lumpur, Malaysia.

1 Supported in part by a grant to Dr. B. Vennesland from the Deutsche Forschungsgemeinschaft and a contribution of the Texas Agricultural Experiment Station.






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Copyright © 1980 by the American Society of Plant Biologists