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Plant Physiology 65:944-948 (1980)
© 1980 American Society of Plant Biologists

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Articles

Synthesis of Nitrate Reductase in Chlorella

II. EVIDENCE FOR SYNTHESIS IN AMMONIA-GROWN CELLS 1

Edward A. Funkhouser

Candadai S. Ramadoss

Department of Plant Sciences, Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas 77843, Forschungsstelle Vennesland der Max-Planck-Gesellschaft, Harnackstrasse 23, D-1000 Berlin 33 (Dahlem), Federal Republic of Germany

Antiserum was prepared against nitrate reductase (EC 1.6.6.1) purified to homogeneity from Chlorella vulgaris Beijerinck. Both crude antiserum and anti-nitrate reductase antibodies prepared from it were used as re-agents to study the synthesis of nitrate reductase. Cell extracts from cultures which were grown with ammonia salts as the sole source of nitrogen contained almost no active enzyme. These extracts did contain material which binds to antibody and is thus immunologically related to purified nitrate reductase. The presence of this cross reacting material in cell extracts was detected by the ability of these extracts to (a) lower the titer of antisera; (b) form a biphasic precipitin curve with purified antibody; and (c) increase the peak height of a standard amount of purified nitrate reductase in rocket immunoelectrophoresis assay. These results suggest that ammonia-grown cells contain nitrate reductase precursor protein.

1 Supported in part by a grant to Dr. B. Vennesland from the Deutsche Forschungsgemeinschaft and a contribution of the Texas Agricultural Experiment Station.






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