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Plant Physiology 65:956-960 (1980)
© 1980 American Society of Plant Biologists

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Articles

ATP Requirement for Mg Chelatase in Developing Chloroplasts 1

Anthony D. Pardo, Barbara M. Chereskin, Paul A. Castelfranco2, Vincent R. Franceschi and Barbara E. Wezelman

Department of Botany, University of California, Davis, California 95616

The synthesis of Mg-protoporphyrin-IX from exogenous protoporphyrin-IX, in a crude plastid pellet extracted from greening cucumber cotyledons was found to require L-glutamate as a cofactor. It has now been shown that glutamate acts in the presence of contaminating mitochondria to provide an ATP regenerating system. With purified plastids, Mg chelatase is not stimulated by glutamate; instead, it requires a high concentration of ATP and is greatly stimulated by added phosphoenolpyruvate and pyruvate kinase. GTP, UTP, CTP, and ITP will not substitute for ATP. ADP in the absence of an ATP generating system is completely ineffective, whereas it is slightly inhibitory in the presence of 10 mM ATP. AMP is strongly inhibitory in the reaction; 50% inhibition is obtained at approximately 3.5 mM AMP in the presence of 10 mM ATP.

2 To whom correspondence should be sent.

1 This research was supported by National Science Foundation Grants PCM-75 10957 and PCM-78 13250.




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A. Kumar Tewari and B. Charan Tripathy
Temperature-Stress-Induced Impairment of Chlorophyll Biosynthetic Reactions in Cucumber and Wheat
Plant Physiology, July 1, 1998; 117(3): 851 - 858.
[Abstract] [Full Text]

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R. Guo, M. Luo, and J. D. Weinstein
Magnesium-Chelatase from Developing Pea Leaves . Characterization of a Soluble Extract from Chloroplasts and Resolution into Three Required Protein Fractions
Plant Physiology, February 1, 1998; 116(2): 605 - 615.
[Abstract] [Full Text] [PDF]


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Copyright © 1980 by the American Society of Plant Biologists