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Plant Physiology 65:1036-1038 (1980)
© 1980 American Society of Plant Biologists

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Articles

Dark Opening of Stomates of Vicia faba in CO2-free Air

EFFECT OF TEMPERATURE ON STOMATAL APERTURE AND POTASSIUM ACCUMULATION 1

Cynthia Rogers

Peter J. H. Sharpe2 and Robert D. Powell3

Department of Biology, Houston Baptist University, Houston, Texas 77074, Texas A&M University System, College Station, Texas 77843

Leaf epidermal strips of Vicia faba were floated on 10 millimolar KCl at temperatures of 10 to 45 C for several time periods in the dark. For the duration of the experiment, a jet of water-saturated, CO2-free air was passed over the strips. The width of the stomatal aperture was measured microscopically and the potassium content was estimated and expressed as the percentage of area of the guard cell pair stained with cobaltinitrite. Stomatal opening in the dark occurred at temperatures above 35 C. The high temperature aperture reduction previously observed at 45 C in the light was not observed in the dark. Potassium accumulation occurred at all temperatures except 10 C, with a maximum at 45 C, which appeared functionally different from that seen previously in the light.


2 Biosystems Research Division, Bioengineering Program.

3 Department of Plant Sciences, Texas Agricultural Experiment Station.

1 This work was supported by the Division of Environmental Biology of the National Science Foundation Grant DEB 77/14408 to the Biosystems Research Division, Department of Industrial Engineering and by the Texas Agricultural Experiment Station, Texas A&M University.







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Copyright © 1980 by the American Society of Plant Biologists