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Plant Physiology 65:1121-1127 (1980)
© 1980 American Society of Plant Biologists

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Articles

Reassociation Kinetics and Cytophotometric Characterization of Peanut (Arachis hypogaea L.) DNA 1

Sukhraj S. Dhillon, Adrian V. Rake2 and Jerome P. Miksche

Department of Botany, North Carolina State University, Raleigh, North Carolina 27650

The base composition of peanut (var. NC-17) DNA determined from thermal denaturation profiles showed an average guanine plus cystosine content of 34% which was in close approximation to 36% guanine plus cytosine calculated from the buoyant density. Buoyant density also indicated the absence of satellite DNA. The genome size, 2.0 x 109 base pairs, as determined by reassociation kinetics of the single copy DNA was close to the genome size determined by cytophotometry, 2.1 x 109 base pairs. Peanut DNA averaging 450 to 600 base pairs long, reassociated in phosphate buffer and fractionated by hydroxylapatite, indicated a DNA genome composition of 36% nonrepetitive or single copy DNA; reassociation in formamide and followed by optical methods indicated the repetitive DNA possesses highly repeated, intermediately repeated and rarely repeated components of DNA with DNA sequences repeated on the average about 38,000, 6,700, and 200 times each. Different criteria of reassociation in formamide revealed further subdivisions of these four separate components of DNA. The DNA of above mentioned NC-17 variety compared to Florigiant variety showed no differences in thermal denaturation profiles, buoyant density, or in genome size.

2 Department of Biology, Wright State University, Dayton, Ohio.

1 Supported by United States Department of Agriculture, Science and Education Administration, research grant 12-14-7001-1154. Paper No. 6175 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, North Carolina.






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Copyright © 1980 by the American Society of Plant Biologists