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Use of High Performance Liquid Chromatography for the Determination of Endogenous Hormone Levels in Solanum tuberosum L. Subjected to Carbon Dioxide Enrichment of the Root Zone ¹

Department of Horticulture and Landscape Architecture, Washington State University, Pullman, Washington 99164

Changes in cytokinin, auxin, and abscisic acid levels were determined by high performance liquid chromatography following CO₂ enrichment of the root zone. Both trans zeatin and zeatin riboside significantly decreased in the roots 6 days after treatment with CO₂. In the leaves zeatin riboside increased whereas trans zeatin showed no significant difference from the control. Indoleacetic acid in the leaves increased dramatically for 6 days following CO₂ treatment; however, indoleacetic acid levels in the roots increased slightly after 2 days and remained higher than the control. Abscisic acid increased shortly after CO₂ treatments; after 6 days this difference was no longer evident.

³ To whom reprint requests should be sent.

¹ Scientific Paper No. 5238. College of Agriculture Research Center, Washington State University, Pullman. Project No. 0321.