Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 66:153-157 (1980)
© 1980 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ho, T.-H. D.
Right arrow Articles by Kleinhofs, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ho, T.-H. D.
Right arrow Articles by Kleinhofs, A.
Agricola
Right arrow Articles by Ho, T.-H. D.
Right arrow Articles by Kleinhofs, A.
Articles

Screening for Barley Mutants With Altered Hormone Sensitivity in Their Aleurone Layers 1

Tuan-Hua David Ho2 and Shu-Cheng Shih

Andris Kleinhofs

Department of Botany, University of Illinois, Urbana, Illinois 61801, Department of Agronomy and Soils and Program in Genetics, Washington State University, Pullman, Washington 99164

A method, based on the diffusion assay of {alpha}-amylase on agar plates, was developed to screen for barley (Himalaya) mutants with altered sensitivity to gibberellic acid (GA3) or abscisic acid (ABA) in their aleurone layers. The seeds produced by sodium azide-mutagenized barley were screened for their ability to synthesize and secrete {alpha}-amylase when treated with different combinations of hormones. Various GA3-insensitive or supersensitive, ABA-insensitive, temperature-dependent GA3-insensitive, and constitutive mutants have been identified. Several stable mutants with altered GA3 sensitivity were recovered. Two of the homozygous GA3-insensitive mutants have been preliminarily characterized. The GA3-enhanced production of {alpha}-amylase and release of phosphatase are hampered in these mutants. However, they have normal stem height, and the uptake of GA3 by their aleurone layers appears to be the same as that of wild-type barley. They are most likely regulatory mutants affecting both {alpha}-amylase synthesis and phosphatase release.

2 To whom reprint requests should be sent.

1 This research was supported by National Science Foundation Grant PCM 78-16143 to T. H. D. H.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1980 by the American Society of Plant Biologists