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Articles

Effect of NAD⁺ on Malate Oxidation in Intact Plant Mitochondria ¹

Physiologie Cellulaire Végétale, Département de Recherche Fondamentale/BV, CEN-G and USM-G 85 X 38041 Grenoble Cedex France

Potato tuber mitochondria oxidizing malate respond to NAD⁺ addition with increased oxidation rates, whereas mung bean hypocotyl mitochondria do not. This is traced to a low endogenous content of NAD⁺ in potato mitochondria, which prove to take up added NAD⁺. This mechanism concentrates NAD⁺ in the matrix space. Analyses for oxaloacetate and pyruvate (with pyruvate dehydrogenase blocked) are consistent with regulation of malate oxidation by the internal NAD⁺/NADH ratio.

³ Present address: Université Scientifique et Technique d'Alger, Algeria.

¹ Supported in part by a grant from the Centre National de la Recherche Scientifique (ERA 847: Interactions Plastes-cytoplasme-mitochondries).

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