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Plant Physiology 66:400-403 (1980)
© 1980 American Society of Plant Biologists

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Isolation of Cytoplasmic Enzymes from Pollen 1

Norman F. Weeden and Leslie D. Gottlieb

Department of Genetics, University of California, Davis, California 95616

The cytoplasmic isozyme of many cytoplasmic-organelle isozyme pairs, as well as other cytoplasmic enzymes in plants, can be readily obtained from pollen by soaking it in an appropriate buffer for 4 hours. Enzymes localized in subcellular organelles appear not to be released during the soaking period, although they are released if the pollen is crushed. The technique is a useful initial step in studies of subcellular localization of enzymes or for obtaining small quantities of cytoplasmic enzymes free of organellar contaminants.

1 The research for this paper was supported by National Research Service Award Grant 5 T32 GM07467 (to N. F. W.) and National Science Foundation Grant DEB 77-08448 (to L. D. G.).




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L. D. Gottlieb
Conservation and Duplication of Isozymes in Plants
Science, April 23, 1982; 216(4544): 373 - 380.
[Abstract] [PDF]


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Copyright © 1980 by the American Society of Plant Biologists