This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (134) Citing Articles via Google Scholar Google Scholar Articles by Foster, J. G. Articles by Hess, J. L. Search for Related Content PubMed PubMed Citation Articles by Foster, J. G. Articles by Hess, J. L. Agricola Articles by Foster, J. G. Articles by Hess, J. L.

Articles

Responses of Superoxide Dismutase and Glutathione Reductase Activities in Cotton Leaf Tissue Exposed to an Atmosphere Enriched in Oxygen ¹

Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

Responses of superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) activities were evaluated in leaf tissue from intact cotton plants (Cotton Branch 1697) which were exposed to 75% O₂, 350 microliters per liter CO₂ for 48 hours. Soluble protein was extracted from O₂-treated and control tissue, and enzyme levels were determined. Superoxide dismutase activity in cotton leaf tissue was high (26 units per milligram protein) under normal conditions of 21% O₂, saturating light, and limiting CO₂, and neither qualitative nor quantitative differences in the cyanide-sensitive or -insensitive forms of the enzyme occurred in response to hyperoxic conditions. Glutathione reductase activity, however, was 2- to 3-fold higher in extracts from tissue exposed to 75% O₂. No increase in activity was observed for the peroxisomal enzymes, glycolate oxidase (EC 1.1.3.1) and catalase (EC 1.11.1.6). Results are consistent with an integrated pathway involving superoxide dismutase and glutathione reductase for protection of sensitive leaf components against detrimental effects of intermediate reduction products of O₂.

³ To whom correspondence should be sent.

¹ This work was supported in part by Cooperative State Research Service Grant 316-15-93 from the United States Department of Agriculture. A preliminary report of this research appeared in Plant Physiol 63: S-609 (1979).

This article has been cited by other articles:

				J. R. Mahan and S. A. Mauget Antioxidant Metabolism in Cotton Seedlings Exposed to Temperature Stress in the Field Crop Sci., September 23, 2005; 45(6): 2337 - 2345. [Abstract] [Full Text] [PDF]

				J. R. Mahan and D. F. Wanjura Seasonal Patterns of Glutathione and Ascorbate Metabolism in Field-Grown Cotton under Water Stress Crop Sci., January 1, 2005; 45(1): 193 - 201. [Abstract] [Full Text] [PDF]

				H. H. Ratnayaka, W. T. Molin, and T. M. Sterling Physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought J. Exp. Bot., October 1, 2003; 54(391): 2293 - 2305. [Abstract] [Full Text] [PDF]

				K. Y. Chiu, C. L. Chen, and J. M. Sung Effect of Priming Temperature on Storability of Primed sh-2 Sweet Corn Seed Crop Sci., November 1, 2002; 42(6): 1996 - 2003. [Abstract] [Full Text] [PDF]

				Y. Jiang and B. Huang Drought and Heat Stress Injury to Two Cool-Season Turfgrasses in Relation to Antioxidant Metabolism and Lipid Peroxidation Crop Sci., March 1, 2001; 41(2): 436 - 442. [Abstract] [Full Text] [PDF]