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Effect of 1-Aminocyclopropane-1-Carboxylic Acid on the Production of Ethylene in Senescing Flowers of Ipomoea tricolor Cav. ¹

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Application of 1-aminocyclopropane-1-carboxylic acid (ACC) to rib segments excised from flowers of Ipomoea tricolor Cav. resulted in the formation of C₂H₄ in greater quantities than produced under natural conditions. The ability of ACC to enhance C₂H₄ production was independent of the physiological age of the tissue and its capacity to synthesize C₂H₄ without applied ACC. When ACC was fed to rib segments that had been treated with [¹⁴C]methionine, incorporation of radioactivity into C₂H₄ was reduced by 80%. Aminoethoxyvinylglycine and aminooxyacetic acid inhibited C₂H₄ production in rib segments of I. tricolor but had no effect on ACC-enhanced C₂H₄ production. Protoplasts obtained from flower tissue of I. tricolor did not form C₂H₄, even when incubated with methionine or selenomethionine. They produced C₂H₄ upon incubation with ACC, however. ACC-dependent C₂H₄ production in protoplasts was inhibited by n-propyl gallate, AgCl, CoCl₂, KCN, Na₂S, and NaN₃. ACC-dependent C₂H₄ synthesis in rib segments and protoplasts was dependent on O₂, the K_m for O₂ being 1.0 to 1.4% (v/v). These results confirm the following pathway for C₂H₄ biosynthesis in I. tricolor. methionine [selenomethionine] S-adenosylmethionine [selenoadenosylmethionine] ACC C₂H₄.

³ Supported, in part, by the Swiss National Science Foundation. Present address: Botanisches Institut, Universität Basel, Schönbeinstr. 6, CH-4056 Basel, Switzerland.

¹ This research was supported by the United States Department of Energy under Contract EY-76-C-02-1338 and by the National Science Foundation through Grant PCM 77-08522 (to H. K.).

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