Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 66:726-730 (1980)
© 1980 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roman, R.
Right arrow Articles by Lark, K. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roman, R.
Right arrow Articles by Lark, K. G.
Agricola
Right arrow Articles by Roman, R.
Right arrow Articles by Lark, K. G.
Articles

Replication of DNA by Nuclei Isolated from Soybean Suspension Cultures 1

Ruth Roman

Michel Caboche2 and Karl G. Lark

Departamento de Bioquímica, Facultad de Quimica, Universidad Nacional Autónoma de Mexico, Z. P. 20, Mexico City, Mexico, Division de Estudios Superiores, Facultad de Quimica, Universidad Nacional Autónoma de Mexico, Z. P. 20, Mexico City, Mexico, Department of Biology, University of Utah, Salt Lake City, Utah 84112

DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of intermediate molecular weight. The characteristics of replication in isolated nuclei correlated well with those of the cells from which they were isolated, as shown by fluorodeoxyuridine synchronization and ultraviolet irradiation experiments.

2 Present address: Laboratoire de biologie cellulaire, Institut National de la Recherche Agronomique, Versailles 78000, Versailles, France.

1 This work was supported by a grant from the North Atlantic Treaty Organization to M. C. and by Grant 10056 from the National Institute of Allergy and Infectious Diseases to K. G. L.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1980 by the American Society of Plant Biologists