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Plant Physiology 66:735-739 (1980)
© 1980 American Society of Plant Biologists

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Articles

Biosynthesis of Ureides from Purines in a Cell-free System from Nodule Extracts of Cowpea [Vigna unguiculata (L) Walp.] 1

K. C. Woo2, Craig A. Atkins and John S. Pate

Department of Botany, University of Western Australia, Nedlands, 6009, Australia

The synthesis of 14C-labeled xanthine/hypoxanthine, uric acid, allantoin, allantoic acid, and urea from [8-14C]guanine or [8-14C]hypoxanthine, but not from [8-14C]adenine, was demonstrated in a cell-free extract from N2-fixing nodules of cowpea (Walp.). The 14C recovered in the acid/neutral fraction was present predominantly in uric acid and allantoin (88-97%), with less than 10% of the 14C in allantoic acid and urea. Time courses of labeling in the cell-free system suggested the sequence of synthesis from guanine to be uric acid, allantoin, and allantoic acid. Ureide synthesis was confined to soluble extracts from the bacteroid-containing tissue, was stimulated by pyridine nucleotides and intermediates of the pathways of aerobic oxidation of ureides, but was completely inhibited by allopurinol, a potent inhibitor of xanthine dehydrogenase (EC 1.2.1.37). The data indicated a purine-based pathway for ureide synthesis by cowpea nodules, and this suggestion is discussed.

2 To whom all correspondence should be addressed at: Department of Environmental Biology, Research School of Biological Sciences, Australian National University, P.O. Box 475, Canberra City, A.C.T, Australia 2601.

1 This work was supported by funds from the Australian Research Grants Committee and the Wheat Industry Research Council of Australia.




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