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Plant Physiology 66:805-808 (1980)
© 1980 American Society of Plant Biologists

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Regulation of Glutamine Synthetase by Light and during Nitrogen Deficiency in Synchronous Chlorella sorokiniana1

Rudolf Tischner

Aloys Hüttermann

Institute of Plant Physiology, University of Göttingen, Untere Karspüle 2, Göttingen, Federal Republic of Germany, Institute of Forest Sciences, University of Göttingen, Untere Karspüle 2, Göttingen, Federal Republic of Germany

A method is described to achieve density labeling of proteins in unicellular algae by using 13CO2. This is a satisfactory procedure especially for work on nitrogen metabolism. The increase in activity of glutamine synthetase (EC 6.3.1.2.) and glutamate synthase (EC 1.4.7.1.) in Chlorella sorokiniana mediated by a dark/light shift and by nitrogen starvation were investigated. Using the method of density labeling and isopycnic centrifugation, we demonstrated that the increase in enzyme activity after a dark/light shift is based on activation rather than de novo synthesis. The increase in enzyme activity after transfer to nitrogen-deficient medium is based both on activation and de novo synthesis.

1 This work was supported by a grant from Deutsche Forschungsgemeinschaft (to R. T.).




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S. El Alaoui, J. Diez, L. Humanes, F. Toribio, F. Partensky, and J. M. García-Fernández
In Vivo Regulation of Glutamine Synthetase Activity in the Marine Chlorophyll b-Containing Cyanobacterium Prochlorococcus sp. Strain PCC 9511 (Oxyphotobacteria)
Appl. Envir. Microbiol., May 1, 2001; 67(5): 2202 - 2207.
[Abstract] [Full Text]


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