Plant Physiology 66:830-834 (1980)
© 1980 American Society of Plant Biologists
Articles
The Site of the Inhibition of the Shikimate Pathway by Glyphosate
II. INTERFERENCE OF GLYPHOSATE WITH CHORISMATE FORMATION IN VIVO AND IN VITRO1
Nikolaus Amrhein2,
Brigitte Deus,
Peter Gehrke and
Hans Christian Steinrücken
Arbeitsgruppe Hormonphysiologie der Pflanzen, Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D-4630 Bochum, Federal Republic Germany
In the presence of the nonselective herbicide glyphosate (N-[phosphonomethyl]glycine), buckwheat (Fagopyrum esculentum Moench) hypocotyls and cultured cells of Galium mollugo L. accumulate an organic acid, which was identified as shikimate by mass-spectroscopy of its methyl ester. After growth in 0.5 millimolar glyphosate for 10 days, G. mollugo cells contained shikimate in amounts of up to 10% of their dry weight. Synthesis of chorismate-derived anthraquinones in G. mollugo was blocked by glyphosate. Chorismate and o-succinylbenzoate (an anthraquinone precursor) alleviated the inhibition. The conclusion drawn from these experiments, that glyphosate inhibits a step in the biosynthetic sequence from shikimate to chorismate, was substantiated by the finding that glyphosate is a powerful inhibitor of the conversion of shikimate to chorismate in cell-free extracts from Aerobacter aerogenes 62-1.
2 To whom reprint requests should be addressed.
1 This work was supported by grants from the Deutsche Forschungsgemeinschaft (to N. A.) and by the Bundesministerium für Forschung und Technologie (to Professor M. H. Zenk).
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