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Plant Physiology 66:1140-1143 (1980)
© 1980 American Society of Plant Biologists

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Utility of Ethidium Bromide in the Extraction from Whole Plants of High Molecular Weight Maize DNA 1

Naomi Kislev and Irwin Rubenstein

Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55108

A procedure is reported for the isolation of high molecular weight maize DNA from whole plant tissue. Nuclei are isolated in the presence of ethidium bromide from leaf, node, and tassel or endosperm tissues and the DNA is extracted and purified. The resulting DNA has a double-strand molecular weight of about 125 kilobase pairs and a single-strand molecular weight of about 125 kilobases. The DNA is cleavable by a number of common restriction endonucleases.

1 This research was supported by Grant GM24756 from the National Institutes of Health and by Grant PCM76-02600 from the National Science Foundation.




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Copyright © 1980 by the American Society of Plant Biologists