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Chloroplast Biogenesis 34

SPECTROFLUOROMETRIC CHARACTERIZATION IN SITU OF THE PROTOCHLOROPHYLL SPECIES IN ETIOLATED TISSUES OF HIGHER PLANTS ¹

Laboratory of Plant Pigment Biochemistry and Photobiology, Department of Horticulture, University of Illinois, Urbana, Illinois 61801

The fluorescence emission and excitation properties of protochlorophyll in etiolated cucumber (Cucumis sativus L.) cotyledons and primary bean (var. Red Kidney) leaves were characterized at 77 K. Contrary to previous studies, it appears that the short-wavelength protochlorophyll emission band consists of four fluorescent components, instead of only one nonphototransformable protochlorophyll. It was demonstrated that etiolated cucumber cotyledons synthesize and accumulate nontransformable protochlorophyll (E₄₄₀, F₆₃₀) as well as short-wavelength phototransformable protochlorophyll (E₄₃₃, F₆₃₃), (E₄₄₄, F₆₃₆), and (E₄₄₅, F₆₄₀). Long-wavelength phototransformable protochlorophyll (E₄₅₀, F₆₅₇) is also formed. In this context, E refers to the Soret excitation maxima and F refers to the red emission maxima of the protochlorophylls.

In etiolated bean leaves, the corresponding species were: nontransformable protochlorophyll (E₄₄₀, F₆₃₀), short-wavelength phototransformable protochlorophylls (E₄₄₁, F₆₃₃), (E₄₄₂, F₆₃₆), and (E₄₄₃, F₆₄₀), and long-wavelength phototransformable protochlorophyll (E₄₄₇, F₆₅₇).

¹ This work was supported by Research Grant PCM 78-11559 from the National Science Foundation and by funds from the Illinois Agricultural Experiment Station.

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