Plant Physiol. Drug Metab Dispos
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Plant Physiology 67:212-215 (1981)
© 1981 American Society of Plant Biologists

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Articles

Effects of Trypsin and Cations on Chloroplast Membranes

Robert C. Jennings, Paolo D. Gerola, Flavio M. Garlaschi and Giorgio Forti

Centro di Studio del Consiglio Nazionale delle Ricerche per la Biologia Cellulare e Molecolare delle Piante, Istituto di Scienze Botaniche, Via Giuseppe Colombo 60, 20133 Milan, Italy

A mild tryptic digestion of chloroplast membranes eliminates the effects of saturating concentrations of cations (3 to 5 millimolar MgCl2) on chlorophyll fluorescence yield, membrane stacking, and photosystem II photochemical efficiency in spinach. At the same time, the negative surface potential of the membranes is increased (by trypsin) as revealed by studies with 9-aminoacridine. High concentrations of cations (25 to 100 millimolar MgCl2) added after trypsin digestion are effective in restoring high fluorescence yields and membrane stacking. High concentrations of cations added after trypsin treatment do not increase the photosystem II efficiency. It is concluded that the "diffuse electrical layer" hypothesis of Barber et al. (Barber J, J Mills, A Love, 1977 FEBS Lett 74: 174-181) satisfactorily explains the effect of trypsin in eliminating the influence of saturating concentrations of cations on chlorophyll fluorescence yield and membrane stacking. However, the effect on photosystem II photochemical efficiency seems to require another mechanism.






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Copyright © 1981 by the American Society of Plant Biologists