This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Hatano, S. Articles by Sadakane, H. Search for Related Content PubMed PubMed Citation Articles by Hatano, S. Articles by Sadakane, H. Agricola Articles by Hatano, S. Articles by Sadakane, H.

Articles

Transition of Lipid Synthesis from Chloroplasts to a Cytoplasmic System during Hardening in Chlorella ellipsoidea^1,2

Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan

Chlorella ellipsoidea Gerneck (IAM C-27) was synchronously grown and cells at an intermediate stage in the ripening phase of the cell cycle were hardened at 3 C for 48 hours. At various times of hardening, the cells were pulse-labeled for 4 minutes with [¹⁴C]NaHCO₃ in the light or with [¹⁴C]glucose in the dark, and the incorporation rate of ¹⁴C into total lipids was determined. A high incorporation rate of [¹⁴C]NaHCO₃ at zero time of hardening decreased after 6 hours. In the next 15 hours, a distinct increase was noted. This increase occurred prior to the development of frost hardiness. Cycloheximide completely inhibited both the increase and the development, and 3-(3,4-dichlorophenyl)-1,1-dimethylurea remarkably lowered the high incorporation rate at zero time. The incorporation rate of [¹⁴C]glucose increased along with hardiness in the dark. These results suggest that the major site of lipid synthesis shifts from chloroplasts to a cytoplasmic system during hardening of Chlorella.

² This is paper No. 6 of the series "Studies on Frost Hardiness in Chlorella ellipsoidea."