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Plant Physiology 67:401-403 (1981)
© 1981 American Society of Plant Biologists

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Articles

Production and Characterization of Antibodies and Establishment of a Radioimmunoassay for Ribosylzeatin 1

Barbara S. Vold2

Nelson J. Leonard3

Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque, New Mexico 87131, Roger Adams Laboratory, School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801

An antibody directed towards ribosyl-trans-zeatin has been produced and characterized. The antiserum was produced in rabbits using ribosyl-zeatin-bovine serum albumin as an immunogen. A radioimmunoassay which employed this antiserum and a tritiated antigen was established. As little as 10 picomoles ribosyl-trans-zeatin could be detected. The specificity of the antiserum was measured in the radioimmunoassay by using nonradioactive nucleosides as competitive inhibitors. Changes in position N6 were more effective in decreasing antibody recognition than changes in position 2. Of particular interest was the interaction of the isomer ribosyl-cis-zeatin. This compound was significantly less active as an inhibitor than ribosyl-trans-zeatin, demonstrating that the antibody was sensitive to minor changes in the structure of the antigen.

The use of this antibody and the radioimmunoassay for ribosylzeatin provides a rapid method for the detection of ribosylzeatin, as well as offering the potential for immunoadsorbent columns which would be useful in the purification of macromolecules, such as tRNA, which contain the ribosylzeatin moiety.

2 Present address: SRI International, Menlo Park, CA 94025.

3 To whom reprint requests should be addressed.

1 This work was supported at the University of New Mexico by Research Grant PM 76-18292 from the National Science Foundation and at the University of Illinois by Research Grant GM-05829 from the National Institutes of Health and by an unrestricted grant from the Hoffmann-LaRoche Foundation.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1981 by the American Society of Plant Biologists