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Plant Physiology 67:460-466 (1981)
© 1981 American Society of Plant Biologists

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Articles

Ethylene Effects in Pea Stem Tissue 1

EVIDENCE OF MICROTUBULE MEDIATION

David A. Steen2 and Arthur V. Chadwick

Department of Biology, Loma Linda University, Loma Linda, California 92354

The marked effects of ethylene on pea stem growth have been investigated. Low temperature and colchicine, both known microtubule depolymerization agents, reverse the effects of ethylene in straight growth tests. Low temperature (6 C) also profoundly reduces the effects of gas in terms of swelling, hook curvature, and horizontal nutation. Deuterium oxide, an agent capable of rigidifying microtubular structure, mimics the effects of ethylene. Electron microscopy shows that microtubule orientation is strikingly altered by ethylene. These findings indicate that some of the ethylene responses may be due to a stabilizing effect on microtubules in plant cells.


2 Present address: Southern Missionary College, Collegedale, TN 37315.

1 Computation assistance was received from the Loma Linda Scientific Computation Facility supported in part by National Institutes of Health Grant RR-276-08.




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Copyright © 1981 by the American Society of Plant Biologists