Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 67:530-534 (1981)
© 1981 American Society of Plant Biologists

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Articles

Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) Cultivars 1

Philip H. McNeil2, Christine H. Foyer and David A. Walker3

Ivan F. Bird, Martin J. Cornelius and Alfred J. Keyes

Agricultural Research Council Research Group on Photosynthesis, Botany Department, University of Sheffield, S10 2TN, United Kingdom, Rothamsted Experimental Station, Harpenden, Hertfordshire, AL5 2JQ, United Kingdom

Partially purified ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) was isolated from diploid and tetraploid cultivars of ryegrass (Lolium perenne L.) using two separate methods. The apparent Km (CO2) values for the enzymes prepared by either method did not differ significantly between diploid and tetraploid when assayed by two separate techniques. The unpurified enzymes from freshly lysed (and fully functional) protoplasts of both diploid and tetraploid cultivars gave virtually identical apparent Km (CO2) values. There was no indication of large differences in affinity for CO2 of illuminated intact protoplasts from the two cultivars.

2 Present address: Shell Research Ltd., Sittingbourne Research Centre, Sittingbourne, Kent ME9 8AG, United Kingdom.

3 To whom all reprint requests should be addressed.

1 Research supported by the United Kingdom Agricultural Research Council and the Rank Prize Fund.






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Copyright © 1981 by the American Society of Plant Biologists