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Plant Physiology 67:565-569 (1981)
© 1981 American Society of Plant Biologists

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Articles

Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes 1

Robert J. Spreitzer2 and Laurens Mets

Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106

A series of non-photoautotrophic mutants of Chlamydomonas reinhardii was isolated by replica-plating mutagenized cells which had been grown in the dark. Many of these acetate-requiring mutants are photosensitive, showing poor growth on acetate medium in the light, but normal growth in the dark. Biochemical characterization showed that the photosensitive mutants all had specific lesions in photosynthesis or photosynthetic pigment accumulation. The acetate-requiring mutants which were not photosensitive were all able to fix CO2. Among the light-sensitive mutants are 15 which show uniparental inheritance. These include six with specific lesions in photosystem II and one with an altered large subunit of ribulose-1,5-bisphosphate carboxylase. Since these two classes of uniparental mutants have been rare or not previously reported, it seems likely that photosensitivity is an important factor which limited their detection in previous mutant isolation experiments.

2 Present address: Agronomy Department, Turner Hall, University of Illinois, Urbana, IL 61801.

1 This work was supported by a grant PCM 78-20684 from the National Science Foundation.




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Copyright © 1981 by the American Society of Plant Biologists