Plant Physiol. Illumina
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Plant Physiology 67:629-632 (1981)
© 1981 American Society of Plant Biologists

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Parameters Influencing the Liposome-Mediated Insertion of Fluorescein Diacetate into Plant Protoplasts

Hirofumi Uchimiya

Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan

Maximum uptake of liposome-encapsulated fluorescein diacetate by Daucus carota protoplasts was observed when 6 x 106 protoplasts per milliliter were incubated with 2.4 x 107 liposomes per milliliter for 1 hour. In the case of Nicotiana glutinosa protoplasts, optimum ratio of protoplasts to liposomes was 1:10, where 2.3 x 105 protoplasts per milliliter were provided. Neutral and positive liposomes were found to be efficient vehicles to transfer their contents into plant protoplasts. When protoplasts treated with liposomes were cultured in a synthetic medium for 1 week, 20% resumed cell divisions.






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Copyright © 1981 by the American Society of Plant Biologists