Plant Physiol.
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Plant Physiology 67:633-639 (1981)
© 1981 American Society of Plant Biologists

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Polygalacturonase from Rhizopus stolonifer, an Elicitor of Casbene Synthetase Activity in Castor Bean (Ricinus communis L.) Seedlings 1

Sung-Chul Lee2 and Charles A. West

Division of Biochemistry, Department of Chemistry, University of California, Los Angeles, California 90024

Apparently homogeneous polygalacturonase-elicitor purified from the filtrates of Rhizopus stolonifer cultures stimulates germinating castor bean seedlings to produce greatly increased levels of casbene synthetase activity. The purification procedure involved gel-filtration chromatography on Sephadex G-25 and G-75 columns followed by cation-exchange chromatography on a Sephadex CM C-50 column. Homogeneity of the purified preparation was indicated by the results of cationic polyacrylamide disc gel electrophoresis and isoelectric focusing (pI = 8.0). The identity of the casbene elicitor activity and polygalacturonase were indicated by the coincidence of the two activities at all stages of purification, the coincidence of both activities with the single protein-staining band detected on a cationic polyacrylamide disc gel and an isoelectric focusing gel, and the identical behavior of both activities on an agarose gel affinity column. The purified polygalacturonase-elicitor is a glycoprotein with approximately 20% carbohydrate content and an estimated molecular weight of 32,000 by polyacrylamide disc gel electrophoresis.

2 Supported as a Cell and Molecular Biology Trainee by National Institutes of Health Training Grant GM-07185 and as a predoctoral trainee by United States Public Health Service Training Grant GM-00463. This work was presented in partial fulfillment of the requirements for the PhD degree from the University of California, Los Angeles, 1980. Present address, Department of Chemistry, University of California-San Diego, La Jolla, CA 92093

1 This work was supported by National Science Foundation Grants 77-14027 and 79-23142.






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Copyright © 1981 by the American Society of Plant Biologists