Plant Physiol. Illumina
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Plant Physiology 67:662-665 (1981)
© 1981 American Society of Plant Biologists

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Articles

Ammonia Assimilation in Alnus glutinosa and Glycine max

SHORT-TERM STUDIES USING [13N]AMMONIUM 1

Karel R. Schubert2 and George T. Coker, III3

Richard B. Firestone4

Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824, Michigan State University Heavy Ion Laboratory, East Lansing, Michigan 48824

The pattern of assimilation of NH4+ by Alnus glutinosa, a N2-fixing, nonleguminous angiosperm, was examined. Detached nodules, roots, and nodulated roots of intact plants were exposed to 13NH4+ for up to 15 minutes. Glutamine was the most highly labeled compound at all times; the only other compound labeled significantly was glutamate. Similar results were obtained after incubating soybean (L. merr) nodules and roots with 13NH4+. These observations and the results of pulse-labeling and inhibitor studies with nodules of Alnus were distinctly different from those predicted for the assimilation of NH4+ via glutamine synthetase and glutamate synthase and suggest that glutamate dehydrogenase may play a major role in the assimilation of exogenously supplied NH4+.

2 To whom reprint requests should be addressed.

3 Supported in part by a National Science Foundation National Needs training grant.

4 Present address: Building 70A-2255, Lawrence Berkeley Laboratory, University of California, Berkeley CA 49720.

1 This research was supported by Grant PCM-77-24 683 from the National Science Foundation and by Grant 5901-0410-9-0248-0 from the United States Department of Agriculture, Science and Education Administration, Competitive Research Grants Office. This is publication 9512 of the Michigan Agricultural Experiment Station.






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Copyright © 1981 by the American Society of Plant Biologists