Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 67:768-773 (1981)
© 1981 American Society of Plant Biologists

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Articles

Rapid Response of Suspension-cultured Parsley Cells to the Elicitor from Phytophthora megasperma var. sojae1

INDUCTION OF THE ENZYMES OF GENERAL PHENYLPROPANOID METABOLISM

Klaus Hahlbrock, Christopher J. Lamb2, Claudio Purwin, Jürgen Ebel, Erich Fautz and Eberhard Schäfer

Biologisches Institute II der Universität, Schänzlestr, 1, D-7800 Freiburg, West Germany

Large and rapid increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase and 4-coumarate:CoA ligase, occurred in suspension-cultured parsley cells (Petroselinum hortense) treated with an elicitor preparation from Phytophthora megasperma var. sojae. Highest enzyme activities were obtained with an elicitor concentration similar to that required for maximal phenylalanine ammonialyase induction in cell suspension cultures of soybean, a natural host of the fungal pathogen.

The changes in phenylalanine ammonia-lyase activity in parsley cells were caused by corresponding changes in the mRNA activity for this enzyme. Phenylalanine ammonia-lyase mRNA activity increased much faster and transiently reached a much higher level in elicitor-treated than in irradiated cell cultures. In contrast to irradiation, treatment of the cells with the elicitor did not induce the enzymes of the flavonoid glycoside pathway, as demonstrated for acetyl-CoA carboxylase and chalcone synthase. Induction of these enzymes by light was abolished by simultaneous application of the elicitor.

2 Permanent address: University of Oxford, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, United Kingdom.

1 This work was supported by Deutsche Forschungsgemeinschaft (SFB 46 and Eb 62/4), Fonds der Chemischen Industrie, and a fellowship from British Council/Deutscher Akademischer Austauschdienst to C. J. L.




This article has been cited by other articles:


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E. Koopmann, E. Logemann, and K. Hahlbrock
Regulation and Functional Expression of Cinnamate 4-Hydroxylase from Parsley
Plant Physiology, January 1, 1999; 119(1): 49 - 56.
[Abstract] [Full Text]

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Rapid Switching of Plant Gene Expression Induced by Fungal Elicitor
Science, March 8, 1985; 227(4691): 1240 - 1243.
[Abstract] [PDF]


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Copyright © 1981 by the American Society of Plant Biologists