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Articles

Sugar Uptake in Lily Pollen ^1,2

A PROTON SYMPORT

Department of Biochemistry, University of Geneva, Geneva, Switzerland, Institute of Biological Chemistry and Graduate Program in Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164

The data presented here are consistent with a proton-sugar co-transport in germinated pollen of Lilium longiflorum Thunb. Optimal uptake occurs at pH 5.0. A K_m of 1.7 to 1.8 millimolar is obtained from the initial rate of pH change induced by sucrose uptake as well as from uptake of [U-¹⁴C]-sucrose. The energy of activation is - 11 kilocalories mole^–1. The effect of several inhibitors and sugar competitors on [U-¹⁴C]sucrose and D-[U-¹⁴C] glucose uptake is given. The possibility of hydrolysis of sucrose prior to its transport into the pollen tube has been considered and reasons for choosing a sucrose-type uptake are presented. The possible in vivo significance of this co-transport process during pollen germination is discussed. Germinated pollen has features to recommend it as an experimental system of choice for studies of sugar uptake.

¹ Dedicated to the memory of Professor Noe Higinbotham.

² This work was supported by Grant GM-22427 from the National Institute of General Medical Sciences, National Institutes of Health, United States Public Health Service, Bethesda, MD, and is Scientific Paper No. 5618, Project 0266, College of Agriculture Research Center, Washington State University, Pullman, WA 99164.

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