Plant Physiology 67:797-801 (1981)
© 1981 American Society of Plant Biologists
Articles
Relationship between Energy-dependent Phosphate Uptake and the Electrical Membrane Potential in Lemna gibba G1 1,2
Cornelia I. Ullrich-Eberius,
Anton Novacky3,
Elke Fischer3 and
Ulrich Lüttge
Institut für Botanik der Technischen Hochschule Darmstadt, Schnittspahnstrasse 3 - 5, D-6100 Darmstadt, Federal Republic of Germany
High rates of phosphate uptake into phosphate-starved Lemna gibba L. G1 were correlated with a high membrane potential (pd = –220 millivolts). In plants maintaining a low pd (–110 millivolts), the uptake rate was only 20% of that of high-pd plants. At the onset of phosphate transport, the membrane of high-pd plants was transiently depolarized. This effect was much smaller in low-pd plants. Light stimulated phosphate uptake and the repolarization upon phosphate-induced depolarization, especially in plants grown without sucrose. The phosphate uptake rate was optimal at pH 6 and decreased with increasing pH, corresponding to the phosphate-induced pd changes. Phosphate starvation stimulated the uptake and increased the phosphate-induced depolarization, thus indicating that phosphate uptake depends on the intracellular phosphate level. It is suggested that uptake of monovalent phosphate in Lemna gibba proceeds by an H+ cotransport dependent on the proton electrochemical potential difference and, hence, on the activity of an H+ -extrusion pump.
3 Permanent address: Department of Plant Pathology, University of Missouri-Columbia, 108 Waters Hall, Columbia, MO 65211.
1 This work was supported by a grant of the Deutsche Forschungsge-meinschaft (to U. L.) and by National Science Foundation Grant PCM 77-25575 (to A.N.).
2 Dedicated to the memory of Noe Higinbotham, our friend and teacher in electrophysiology.
This article has been cited by other articles:
|
|
|
|
 
J. P. Hammond and P. J. White
Sucrose transport in the phloem: integrating root responses to phosphorus starvation
J. Exp. Bot.,
January 1, 2008;
59(1):
93 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shen, J. Chen, Z. Wang, C. Yang, T. Sasaki, Y. Yamamoto, H. Matsumoto, and X. Yan
Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation
J. Exp. Bot.,
March 1, 2006;
57(6):
1353 - 1362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Rubio, A. Linares-Rueda, M. J. Garcia-Sanchez, and J. A. Fernandez
Physiological evidence for a sodium-dependent high-affinity phosphate and nitrate transport at the plasma membrane of leaf and root cells of Zostera marina L.
J. Exp. Bot.,
February 1, 2005;
56(412):
613 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Scheffer, S. Szabo, A. Gragnani, E. H. van Nes, S. Rinaldi, N. Kautsky, J. Norberg, R. M. M. Roijackers, and R. J. M. Franken
Floating plant dominance as a stable state
PNAS,
April 1, 2003;
100(7):
4040 - 4045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Facanha and A. L. Okorokova-Facanha
Inhibition of Phosphate Uptake in Corn Roots by Aluminum-Fluoride Complexes
Plant Physiology,
August 1, 2002;
129(4):
1763 - 1772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. K. Varadarajan, A. S. Karthikeyan, P. D. Matilda, and K. G. Raghothama
Phosphite, an Analog of Phosphate, Suppresses the Coordinated Expression of Genes under Phosphate Starvation
Plant Physiology,
July 1, 2002;
129(3):
1232 - 1240.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. S. Muchhal and K. G. Raghothama
Transcriptional regulation of plant phosphate transporters
PNAS,
May 11, 1999;
96(10):
5868 - 5872.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Liu, U. S. Muchhal, M. Uthappa, A. K. Kononowicz, and K. G. Raghothama
Tomato Phosphate Transporter Genes Are Differentially Regulated in Plant Tissues by Phosphorus
Plant Physiology,
January 1, 1998;
116(1):
91 - 99.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Mitsukawa, S. Okumura, Y. Shirano, S. Sato, T. Kato, S. Harashima, and D. Shibata
Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions
PNAS,
June 24, 1997;
94(13):
7098 - 7102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
