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Plant Physiology 67:962-964 (1981)
© 1981 American Society of Plant Biologists

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Articles

Use of Tetraethylthiuram Disulfide to Discriminate between Alternative Respiration and Lipoxygenase 1

Marna Geyer Miller and Ralph L. Obendorf

Department of Agronomy, New York State College of Agriculture and Life Sciences, Cornell University, Ithaca, New York 14853

Mitochondria from axes of Glycine max (L.) Merr. cv. Chippewa 64 seedlings purified on discontinuous Percoll gradients exhibited classical cyanide-resistant respiration. These mitochondria also possessed lipoxygenase activity, as determined by O2 uptake in the presence of 0.8 millimolar linoleic acid. This activity is inhibited by most known inhibitors of alternative respiration (i.e. hydroxamates and propyl gallate). Tetraethylthiuram disulfide (disulfiram) at 50 micromolar inhibited cyanide-resistant succinate oxidation by 90 per cent, whereas concentrations as high as 100 micromolar had no effect on lipoxygenase activity. Use of tetraethylthiuram disulfide allows discrimination between alternative respiration and lipoxygenase activity in mitochondria.

1 Agronomy Department Series Paper 1368, New York State College of Agriculture and Life Sciences, Cornell University, Ithaca, New York.






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Copyright © 1981 by the American Society of Plant Biologists