This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Simpson, E. Articles by Davies, D. D. Search for Related Content PubMed PubMed Citation Articles by Simpson, E. Articles by Davies, D. D. Agricola Articles by Simpson, E. Articles by Davies, D. D.

Articles

Measurement of Protein Degradation in Leaves of Zea mays Using [³H]Acetic Anhydride and Tritiated Water

Robert J. Cooke² and David D. Davies²

Department of Biology, Washington University, St. Louis, Missouri 63130, School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom

The rate of protein degradation in Zea mays leaves has been estimated by using tritiated water and [³H]acetic anhydride as the labeling agents. Both methods circumvent many of the problems usually associated with measuring protein degradation in plants. The half-life of ribulose-1,5-bisphosphate carboxylase protein in second leaves of 13-day-old seedlings under continuous light was found to be 7.8 ± 0.9 days by the tritiated water technique and 6.5 ± 0.8 days by the [³H]acetic anhydride method. The half-lives determined under a 14-hour-light, 10-hour-dark photoperiod are 6.2 ± 0.8 days with tritiated water and 5.4 ± 0.4 days with [³H]acetic anhydride. Whereas the values obtained by the two methods do not differ significantly, the use of either method for the determination of protein half-life can be recommended.

² Partly supported by Grant Ag 83/14 from the Agricultural Research Council.

This article has been cited by other articles:

				C. A. Lehmeier, F. A. Lattanzi, R. Schaufele, M. Wild, and H. Schnyder Root and Shoot Respiration of Perennial Ryegrass Are Supplied by the Same Substrate Pools: Assessment by Dynamic 13C Labeling and Compartmental Analysis of Tracer Kinetics Plant Physiology, October 1, 2008; 148(2): 1148 - 1158. [Abstract] [Full Text] [PDF]

				A.H. Kingston-Smith and C.H. Foyer Bundle sheath proteins are more sensitive to oxidative damage than those of the mesophyll in maize leaves exposed to paraquat or low temperatures J. Exp. Bot., January 1, 2000; 51(342): 123 - 130. [Abstract] [Full Text] [PDF]