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Effect of Temperature on Proton Efflux from Isolated Chloroplast Thylakoids

Biology Department, Georgia State University, University Plaza, Atlanta, Georgia 30303, Laboratory for Microbial and Biochemical Sciences, Georgia State University, University Plaza, Atlanta, Georgia 30303

Temperature-induced changes in the decay of the light-induced proton gradient of chloroplast thylakoids isolated from chilling-resistant and chilling-sensitive plants have been examined. In the presence of N-methylphenazonium methosulfate, the thylakoids isolated from chilling-resistant barley (cv. Kanby) and pea (cv. Alaska) and chilling-sensitive mung bean (cv. Berken) plants showed temperature-induced changes at approximately 8.6, 13.3, and 14.0 C, respectively. Barley thylakoids assayed in the presence of sodium thiocyanate also showed a change at 8.6 C, whereas with no addition or upon the inclusion of both N-methylphenazonium methosulfate and sodium thiocyanate the change occurred at approximately 11.5 C.

Temperature-induced changes in electron transport activities occurred at temperatures approximating those for proton efflux for each of the three plants. These results indicate that temperature has a general effect on thylakoid membranes and that chloroplast thylakoids from chilling-resistant and chilling-sensitive plants have their proton permeability properties affected similarly by temperature.