Plant Physiology 68:65-69 (1981)
© 1981 American Society of Plant Biologists
Articles
Localization of Enzymes of Ureide Biosynthesis in Peroxisomes and Microsomes of Nodules 1
Joanna F. Hanks,
N. E. Tolbert and
Karel R. Schubert
Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824
The intracellular location of enzymes involved in the synthesis of the ureides, allantoin and allantoic acid, was investigated in nodules of Glycine max L. Merr. Cellular organelles were separated on isopycnic sucrose density gradients. Xanthine dehydrogenase activity (270 nanomoles per min per gram fresh weight) was totally soluble, whereas approximately 15% of the total uricase and catalase activities (1 and 2000 micromoles per minute per gram fresh weight, respectively) was in the fraction containing intact peroxisomes. Allantoinase activity (680 nanomoles per minute per gram fresh weight) was associated with the microsomal fraction, which apparently originates from the endoplasmic reticulum.
1 Research supported in part by grants from the National Science Foundation to N. E. T. (PCM 78 15891) and United States Department of Agriculture grant (5901-0410-9-0248-0) to K. R. S. J. F. H was supported by a Graduate Professional Opportunity Program Fellowship from the National Institutes of Health. Published as journal article 9647 of the Michigan Agricultural Experiment Station. A preliminary report has been published (9).
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