Plant Physiol.
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Plant Physiology 68:88-92 (1981)
© 1981 American Society of Plant Biologists

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Articles

The Appearance of New Active Forms of Trypsin Inhibitor in Germinating Mung Bean (Vigna radiata) Seeds 1

Elke Lorensen, Regina Prevosto and Karl A. Wilson2

The Department of Biological Sciences and Center for Somatic-Cell Genetics and Biochemistry, State University of New York at Binghamton, Binghamton, New York 13901

Ungerminated seeds of mung bean contain a single major species (F) of trypsin inhibitor with five minor species (A-E) separable on diethylaminoethyl-cellulose. During germination the level of trypsin inhibitory activity decreases from 1.8 units/grams dry weight in ungerminated cotyledons to 1.2 units/grams in cotyledons from seeds germinated 5 days. This decrease is accompanied by major changes in the distribution of inhibitory activity among the inhibitor species. By 48 hours of germination, inhibitor F has largely disappeared with an accompanying rapid increase in inhibitor C. Similarly, though less rapidly, inhibitor E decreases while inhibitor A increases. A similar sequence of changes is found in vitro when purified inhibitor F is incubated with extracts from seeds germinated 96 hours. The combined in vivo and in vitro data suggest a conversion sequence of: F -> E -> C -> A. The in vitro conversion is inhibited by phenylmethyl sulfonyl fluoride but not by iodoacetamide, indicating that at least the initial phases of inhibitor conversion are not catalyzed by the mung bean vicilin peptidohydrolase.

2 To whom correspondence should be directed.

1 Supported by UAC grant-in-aid 7412A from the Research Foundation of State University of New York and Grant PCM-8003854 from the National Science Foundation.






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