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Identification of a Dihydrophaseic Acid Aldopyranoside from Soybean Tissue ¹

³ Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108, ⁴ Department of Horticultural Science and Landscape Architecture, University of Minnesota, St. Paul, Minnesota 55108, ⁵ Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55108

A previously unidentified abscisic acid metabolite has been isolated and characterized. (±)-[2-¹⁴C]Abscisic acid was incubated in intact soybean leaves and pods; the radiolabeled metabolite was purified by high performance liquid chromatography with on-line scintillation spectrometry detection. Gas chromatography-mass spectrometry was used to obtain spectra of the acetylated and methyl esterified derivatives. The data were consistent with a proposed dihydrophaseic acid-aldopyranoside identity. Conjugation through the 4'-hydroxyl of dihydrophaseic acid is suggested.

¹ Contribution from the Agricultural Experiment Station, University of Minnesota, St. Paul, MN 55108. Paper No. 11,398, Scientific Journal Series. This research was supported in part by the Science and Education Administration of the United States Department of Agriculture under Grant 5901-0410-8-0183-0 from the the Competitive Research Grants Office. This research was also supported in part by a grant from the Minnesota Soybean Research and Promotion Council.