This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Morohashi, Y. Articles by Yeung, E. C. Search for Related Content PubMed PubMed Citation Articles by Morohashi, Y. Articles by Yeung, E. C. Agricola Articles by Morohashi, Y. Articles by Yeung, E. C.

Articles

Biogenesis of Mitochondria in Imbibed Peanut Cotyledons ¹

II. DEVELOPMENT OF LIGHT AND HEAVY MITOCHONDRIA

Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4 Canada

There are two types of mitochondria present in imbibed peanut cotyledons: a light type (density 1.182 grams per cubic centimeter) and a heavy type (density 1.205 grams per cubic centimeter). The membrane fractions from these two types can be distinguished using sucrose density gradient analysis, and differences in membrane density between the light and heavy types are reflected in differences in their protein N and phospholipid P composition. With increasing time after imbibition, there is a substantial increase in the amount and activity of the light type of mitochondria due to their de novo synthesis. The membrane density of the light mitochondrial fraction declines over 5 days after the start of imbibition as the phospholipid P to protein N ratio increases. The heavy mitochondrial fraction declines during the first 3 days after the start of imbibition, and then it remains at a low, but constant, level thereafter. Even during the decline, however, there is synthesis of proteins comparable to that into light mitochondria. The mitochondrial biogenesis that has been observed in peanut cotyledons is of the light type, the function and physiological importance of the minor heavy type is not known.

¹ Supported by Natural Sciences and Engineering Research Council Grants A6352 and E2550 (to J. D. B.) and A6704 (to E. C. Y.).

This article has been cited by other articles:

				D. C. Logan The mitochondrial compartment J. Exp. Bot., March 1, 2006; 57(6): 1225 - 1243. [Abstract] [Full Text] [PDF]

				A. H. Millar, A. E. Trend, and J. L. Heazlewood Changes in the Mitochondrial Proteome during the Anoxia to Air Transition in Rice Focus around Cytochrome-containing Respiratory Complexes J. Biol. Chem., September 17, 2004; 279(38): 39471 - 39478. [Abstract] [Full Text] [PDF]

				D. C. Logan, A. H. Millar, L. J. Sweetlove, S. A. Hill, and C. J. Leaver Mitochondrial Biogenesis during Germination in Maize Embryos Plant Physiology, February 1, 2001; 125(2): 662 - 672. [Abstract] [Full Text]